# HiSeq2000 - Next Level Hacking **URL:** \ **Category:** ReSeq\ **Created:** [February 2, 2018, 11:34am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325 "2018-02-02T11:34:05Z")\ **Posts on this page:** 20\ **Page:** 10
**Author:** ![jmarkham](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/jmarkham/32/508_2.png) [@jmarkham](https://forum.hackteria.org/u/jmarkham)\ **Post date:** [April 2, 2019, 1:48pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/182 "2019-04-02T13:48:10Z")
I didn’t get it but I can’t see it in spam so perhaps I didn’t subscribe. I posted to the mm list, which was the source of that guy I put you in touch with who has imageJ experience. How does one get to the gosht thing? Or is it for organisers and the like? John ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [April 2, 2019, 2:16pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/183 "2019-04-02T14:16:54Z")
Everyone who is interested in open science hardware is welcome! (Though the day might be moved to next week now.) More details [here](https://forum.openhardware.science/t/april-gosh-community-call-join-us/1732/1). It’s not needed to join the call but you should also join the GOSH forum @jmarkham. I am sure you will find other discussions that interest you there. ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [April 10, 2019, 1:02pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/184 "2019-04-10T13:02:37Z")
I sent out another email. Hopefully it reached people’s inboxes this time? We are having the GOSH call tomorrow on [this Jitsi](https://meet.kitspace.org/gosh) at [4pm CEST](https://www.timeanddate.com/worldclock/fixedtime.html?msg=GOSH+April+Community+Call&iso=20190411T16&p1=87&ah=1) and I’ll spend 15 mins on ReSeq. ---
**Author:** ![dusjagr](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/dusjagr/32/24_2.png) [@dusjagr](https://forum.hackteria.org/u/dusjagr)\ **Post date:** [April 14, 2019, 8:44pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/185 "2019-04-14T20:44:42Z")
just found this on the forum here.,… [https://forum.hackteria.org/t/pgm-sequenser-reverce-engineering/](https://forum.hackteria.org/t/pgm-sequenser-reverce-engineering/) ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [April 17, 2019, 10:31am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/186 "2019-04-17T10:31:07Z")
Did everyone get the mail titled “ReSeq: Reusing the HiSeqs Campaign” that I sent on the 10th? @dusjagr @mamaya @gaudi @tamberg Getting an error when visiting on our page at the moment. 😲 I notified wemakeit. ---
**Author:** ![dusjagr](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/dusjagr/32/24_2.png) [@dusjagr](https://forum.hackteria.org/u/dusjagr)\ **Post date:** [April 17, 2019, 10:41am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/187 "2019-04-17T10:41:56Z")
> [@kaspar](#): > > hen visiting on our page at the moment. 😲 I notified wemakeit. no i didnt receive it… ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [April 17, 2019, 1:22pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/188 "2019-04-17T13:22:21Z")
Sent out another one, let me know. ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [April 17, 2019, 3:03pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/189 "2019-04-17T15:03:00Z")
Oops, @dusjagr, I don’t think you actually signed up to the list. Thought you had… let’s wait to hear from others wether they got the messages. ---
**Author:** ![bengtsjolen](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/bengtsjolen/32/469_2.png) [@bengtsjolen](https://forum.hackteria.org/u/bengtsjolen)\ **Post date:** [April 17, 2019, 3:56pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/190 "2019-04-17T15:56:46Z")
Hey, I got the: “ReSeq: Reusing the HiSeqs Campaign” today. Too bad wemakeit was down though ☹ Btw, speaking to National Genomics Infrastructure in Sweden today about a sequencing project, and also mentioning Reseq, I was immediately offered 1 of their 2 Pacbio RS II systems if I just come and get it 😉 Weighs 1 ton apparently… similar story as with the Hiseq systems, service contracts running out, too expensive to use and there are better methods now: Nanopore PromethION, PacBio HiFi etc. /b ---
**Author:** ![dusjagr](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/dusjagr/32/24_2.png) [@dusjagr](https://forum.hackteria.org/u/dusjagr)\ **Post date:** [April 17, 2019, 6:34pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/191 "2019-04-17T18:34:38Z")
it’s back online… and i think with 2 more weeks to go, we (you) are doing good. Now for a full sprint second wave of promo and you’ll get to reach the target. gonna ping my silicon valley friends again. greets, marc ---
**Author:** ![jmarkham](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/jmarkham/32/508_2.png) [@jmarkham](https://forum.hackteria.org/u/jmarkham)\ **Post date:** [April 21, 2019, 1:50pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/192 "2019-04-21T13:50:26Z")
@kasper, I didn’t get it. ---
**Author:** ![jmarkham](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/jmarkham/32/508_2.png) [@jmarkham](https://forum.hackteria.org/u/jmarkham)\ **Post date:** [April 21, 2019, 2:00pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/193 "2019-04-21T14:00:33Z")
@bengtsjolen, what’s your project? There’s some good stuff inside those pacbios. I dunno what weighs so much though. Maybe you can start an indoor [pick-a-part](https://www.pick-a-part.com.au/). ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [April 30, 2019, 2:22pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/194 "2019-04-30T14:22:56Z")
3 days left and 117 CHF to go! > **[ReSeq: Reuse DNA Sequencers](https://wemakeit.com/projects/reseq-reuse-dna-sequencers?locale=en)** > > We want to reuse older DNA sequencers and turn them into advanced instruments for more general scientific research. Help us develop software so everyone can repurpose and avoid wasting these machines. ---
**Author:** ![mamaya](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/mamaya/32/1025_2.png) [@mamaya](https://forum.hackteria.org/u/mamaya)\ **Post date:** [April 30, 2019, 2:49pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/195 "2019-04-30T14:49:14Z")
I think you all are crazy and you will get nobel prizes if you do this project globally, ^^ Now I want one of this machines too!! Imagine all the Artfotography you could do?! Maya Minder ---
**Author:** ![kaspar](https://avatars.discourse-cdn.com/v4/letter/k/5f8ce5/32.png) [@kaspar](https://forum.hackteria.org/u/kaspar)\ **Post date:** [May 1, 2019, 10:18am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/196 "2019-05-01T10:18:48Z")
We made it to our goal and the funds were doubled! 🥳 Maybe as a celebration we can make a new ReSeq section in this forum as you suggested before @dusjagr. ---
**Author:** ![dusjagr](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/dusjagr/32/24_2.png) [@dusjagr](https://forum.hackteria.org/u/dusjagr)\ **Post date:** [May 1, 2019, 10:24am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/197 "2019-05-01T10:24:03Z")
done. so here is a dedicated category. m [https://forum.hackteria.org/c/reseq](https://forum.hackteria.org/c/reseq) ---
**Author:** ![jmarkham](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/jmarkham/32/508_2.png) [@jmarkham](https://forum.hackteria.org/u/jmarkham)\ **Post date:** [May 1, 2019, 1:06pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/198 "2019-05-01T13:06:03Z")
Imagine my surprise and joy! Well done everyone. Coincidentally I’ve just come back from work where I had to clad the franken-seq in Coreflute to make it removable, if necessary, from the PC2 lab where it now lives. ![image](https://canada1.discourse-cdn.com/flex030/uploads/hackteria/original/1X/aacd3e48e747a514f96a54def7a1a8c905552451.jpeg) The cladding will need to be moved in order to do any surgery on the internals which is a pain. If we get something working and into the MM distribution then we should do some promotional work. At a minimum, write a paper which then gives other media something to refer to. For example like the flypi guys did: [https://www.nature.com/news/open-hardware-pioneers-push-for-low-cost-lab-kit-1.19518](https://www.nature.com/news/open-hardware-pioneers-push-for-low-cost-lab-kit-1.19518) [http://blogs.nature.com/naturejobs/2017/04/19/techblog-need-an-instrument-build-it/](http://blogs.nature.com/naturejobs/2017/04/19/techblog-need-an-instrument-build-it/) I don’t know if anyone has noticed but MM has some Arduino integration. [https://micro-manager.org/wiki/Arduino](https://micro-manager.org/wiki/Arduino) [https://micro-manager.org/wiki/ArduinoNeoPixel](https://micro-manager.org/wiki/ArduinoNeoPixel) That second one drives X,Y,Z stepper motors and lighting. I was considering motorising and automating an old Olympus scope that I have. ---
**Author:** ![lorbar](https://avatars.discourse-cdn.com/v4/letter/l/dbc845/32.png) [@lorbar](https://forum.hackteria.org/u/lorbar)\ **Post date:** [August 31, 2019, 12:31pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/199 "2019-08-31T12:31:43Z")
Hi! my name is Lorenzo and I’m a researcher in Stockholm. This is a great project, and stunning results! I was interested in repurposing the GAIIx illumina seq as the Burge lab did ([https://www.nature.com/articles/nbt.1882](https://www.nature.com/articles/nbt.1882)) and I’m sure you are well aware of, for biophysics experiments. I was wondering if you think this would be feasible on the HiSeq 2000 as well or if it’s something you wanted to implement beside fluorescence imaging. @bengtsjolen I see you are in Stockholm? Would you mind meeting sometimes? I read you contacted someone from the NGI and they had some machines to give away? Thanks! Lorenzo ---
**Author:** ![jmarkham](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/jmarkham/32/508_2.png) [@jmarkham](https://forum.hackteria.org/u/jmarkham)\ **Post date:** [September 1, 2019, 11:50pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/200 "2019-09-01T23:50:22Z")
Hi Lorenzo, it should be possible to do other kinds of experiments from within micro manager. It just needs DeviceAdaptors to be written for the devices controlled via the many serial ports. (DeviceAdaptors are C++ classes in MM that handle hardware abstraction and act as device drivers). Then the hardware can be controlled by Beanshell scripts to implement protocols from within MM. See here ([https://micro-manager.org/wiki/Micro-Manager\_Programming\_Guide](https://micro-manager.org/wiki/Micro-Manager_Programming_Guide)) for examples. In an ideal world someone would also write java plugins to provide user interface elements, as has been done for the microscope functionality. For example, for trouble shooting I think it would be useful to have a panel showing the status of everything. John ---
**Author:** ![mamaya](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.hackteria.org/mamaya/32/1025_2.png) [@mamaya](https://forum.hackteria.org/u/mamaya)\ **Post date:** [September 4, 2019, 8:25am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/201 "2019-09-04T08:25:55Z")
Hello There This is a bit short in Time, but there will be a next gathering at the gaudilabs for further research and hacks from the 20th - 27th of September and a first stunning appereance of the HiSeq as Mikroskopic Camera device at the public scientific photo contest in the Zoo of Zurich on the 27th September. (public opencall for participation) Kaspar will be here from the 20th - 24th and if any of you would like to join, pls feel welcome, also in remote. All best Maya Maya All best [Previous page](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325.md?page=9) [Next page](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325.md?page=11)