# HiSeq2000 - Next Level Hacking

**URL:** <https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325>\
**Category:** ReSeq\
**Created:** [February 2, 2018, 11:34am UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325 "2018-02-02T11:34:05Z")\
**Posts on this page:** 1\
**Showing post:** 203

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**Author:** ![kunal](https://avatars.discourse-cdn.com/v4/letter/k/dec6dc/32.png) [@kunal](https://forum.hackteria.org/u/kunal)\
**Post date:** [September 20, 2019, 3:25pm UTC](https://forum.hackteria.org/t/hiseq2000-next-level-hacking/325/203 "2019-09-20T15:25:11Z")

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Hi All,

I’ve been working on controlling a HiSeq2500 and this forum has been an immensely helpful resource.

I mainly want control of the instrument to do some spatial transcriptomics or proteomic imaging.

I successfully got images from the camera using TDIscan and the FPGA/Y-STAGE commands.

However I’d like to use the DCAM API instead of TDIscan.

I can talk to and configure the cameras with the DCAM API, but the cameras don’t seem to get triggered after using the FPGA/Y-STAGE.

The cameras report taking 0 frames after arming the TDI and moving the Y-STAGE.

Has anyone been successful in getting scans with the DCAM API instead of TDIscan?

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