HiSeq2000 - Next Level Hacking

Hi All,

I’ve been working on controlling a HiSeq2500 and this forum has been an immensely helpful resource.

I mainly want control of the instrument to do some spatial transcriptomics or proteomic imaging.

I successfully got images from the camera using TDIscan and the FPGA/Y-STAGE commands.

However I’d like to use the DCAM API instead of TDIscan.

I can talk to and configure the cameras with the DCAM API, but the cameras don’t seem to get triggered after using the FPGA/Y-STAGE.

The cameras report taking 0 frames after arming the TDI and moving the Y-STAGE.

Has anyone been successful in getting scans with the DCAM API instead of TDIscan?