Everyone who is interested in open science hardware is welcome! (Though the day might be moved to next week now.) More details here. It’s not needed to join the call but you should also join the GOSH forum @jmarkham. I am sure you will find other discussions that interest you there.
I got the: “ReSeq: Reusing the HiSeqs Campaign” today. Too bad wemakeit
was down though
Btw, speaking to National Genomics Infrastructure in Sweden today about
a sequencing project, and also mentioning Reseq, I was immediately
offered 1 of their 2 Pacbio RS II systems if I just come and get it
Weighs 1 ton apparently… similar story as with the Hiseq systems,
service contracts running out, too expensive to use and there are better
methods now: Nanopore PromethION, PacBio HiFi etc.
it’s back online… and i think with 2 more weeks to go, we (you) are doing good. Now for a full sprint second wave of promo and you’ll get to reach the target.
gonna ping my silicon valley friends again.
@bengtsjolen, what’s your project? There’s some good stuff inside those pacbios. I dunno what weighs so much though.
Maybe you can start an indoor pick-a-part.
Imagine my surprise and joy! Well done everyone. Coincidentally I’ve just come back from work where I had to clad the franken-seq in Coreflute to make it removable, if necessary, from the PC2 lab where it now lives.
my name is Lorenzo and I’m a researcher in Stockholm. This is a great project, and stunning results!
I was interested in repurposing the GAIIx illumina seq as the Burge lab did (https://www.nature.com/articles/nbt.1882) and I’m sure you are well aware of, for biophysics experiments.
I was wondering if you think this would be feasible on the HiSeq 2000 as well or if it’s something you wanted to implement beside fluorescence imaging.
@bengtsjolen I see you are in Stockholm? Would you mind meeting sometimes? I read you contacted someone from the NGI and they had some machines to give away?
Hi Lorenzo, it should be possible to do other kinds of experiments from within micro manager. It just needs DeviceAdaptors to be written for the devices controlled via the many serial ports. (DeviceAdaptors are C++ classes in MM that handle hardware abstraction and act as device drivers). Then the hardware can be controlled by Beanshell scripts to implement protocols from within MM. See here (https://micro-manager.org/wiki/Micro-Manager_Programming_Guide) for examples. In an ideal world someone would also write java plugins to provide user interface elements, as has been done for the microscope functionality. For example, for trouble shooting I think it would be useful to have a panel showing the status of everything.
This is a bit short in Time, but there will be a next gathering at the gaudilabs for further research and hacks from the 20th - 27th of September and a first stunning appereance of the HiSeq as Mikroskopic Camera device at the public scientific photo contest in the Zoo of Zurich on the 27th September. (public opencall for participation) Kaspar will be here from the 20th - 24th and if any of you would like to join, pls feel welcome, also in remote.
All best
Maya
Maya