Identify suitable insertion site or ideally a Safe Harbour Site for a CRISPR/Cas9
Knock-In experiment in a specific plant species to be shared under NDA.
Subsequently we need assistance in designing a suitable gRNA construct to target the identified site.
Insertion site has to provide for:
- Rubust and stable expression of knock-in gene
- Minimal perturbation of endogenous gene activity
Knowledge of host plants’ genome sequence is currently publicly available Genbank information.
Additional sequencing efforts can be considered. Those interested, please send your CV via email to firstname.lastname@example.org or email@example.com